Genetically Encoded Cyclopropene Directs Rapid, Photoclick-Chemistry-Mediated Protein Labeling in Mammalian Cells. Angewandte Chemie International Edition，20 SEP 2012

Angewandte Chemie International Edition，*Article first published online: 20 SEP 2012，doi: 10.1002/anie.201205352

Genetically Encoded Cyclopropene Directs Rapid, Photoclick-Chemistry-Mediated Protein Labeling in Mammalian Cells

Dr. Zhipeng Yu2,？, Yanchao Pan1,？, Dr. Zhiyong Wang2, Prof. Dr. Jiangyun Wang1,*, Prof. Dr. Qing Lin2,*Article first published online: 20 SEP 2012

Author Information

1Laboratory of Noncoding RNA, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 (P.R. China)

2Department of Chemistry, State University of New York at Buffalo, Buffalo, NY 14260 (USA)

？These two authors contributed equally to the work.

Email: Prof. Dr. Jiangyun Wang (jwang@ibp.ac.cn), Prof. Dr. Qing Lin (qinglin@buffalo.edu)

*Laboratory of Noncoding RNA, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 (P.R. China)

Abstract

The introduction of bioorthogonal organic reporters into proteins site-selectively through genetic,1 metabolic,2 or enzyme-catalyzed ligation methods,3 in conjunction with a growing repertoire of bioorthogonal reactions,4 has allowed for the visualization and regulation of proteins in their native environment.5 Numerous small organic functional groups, such as ketones,6 azides,7 terminal alkynes,8 and terminal alkenes,9 as well as larger reactive bioorthogonal groups, such as cyclooctyne,10 norbornene,11 transcyclooctene,10b,？11b tetrazole,12 and tetrazine13 have been genetically encoded for site-selective protein labeling in vivo. To track fast protein dynamics in vivo, it is imperative that these genetically encoded bioorthogonal reporters direct fast and selective bioorthogonal labeling with the cognate biophysical probes, preferably with spatiotemporal control.

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全文链接：http://onlinelibrary.wiley.com/doi/10.1002/anie.201205352/abstract