Genetic Incorporation of a Metal-Chelating Amino Acid as a Probe for Protein Electron Transfer. Angew Chem Int Ed Engl. 2012 Aug 31.

Angewandte Chemie International Edition, 2012 Aug 31. doi: 10.1002/anie.201204962.

Genetic Incorporation of a Metal-Chelating Amino Acid as a Probe for Protein Electron Transfer.

Liu X, Li J, Dong J, Hu C, Gong W, Wang J.

Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing, 100101 (China).

Abstract

ET encounters jellyfish: Through the incorporation of the metal-chelating amino acid pyTyr into green fluorescent protein (GFP) from jellyfish, photoinduced electron transfer (ET) from the GFP chromophore to a bound Cu(II) ion was shown to occur within one nanosecond in a distance dependent manner. The crystal structure of GFP with pyTyr at a specific position shows the structural basis for the nanomolar binding affinity of pyTyr to Cu(II) ions.

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