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中科院青促会会员

王久宇  学士 副研究员  

中国科学院青年创新促进会会员 
中科院生物物理所,中国科学院核酸生物学重点实验室

研究方向:CRISPR-Cas系统的结构生物学研究

电子邮件:wangjiuyu@ibp.ac.cn

电       话:010-64884212

通讯地址:北京市朝阳区大屯路15号(100101)

英文版个人网页:http://english.ibp.cas.cn/faculty/index_18316.html?json=http://www.ibp.cas.cn/sourcedb_ibp_cas/cn/ibpexport/EN_zkyqchhy/202005/t20200519_5582894.json

 

简       历:

  2007 - 2011  中国农业大学 生物学院 理学学士

  2011 - 2015  中国科学院生物物理所 研究实习员

  2015 - 至今  中国科学院生物物理研究所 副研究员

获奖及荣誉:

 

社会任职:

 

研究方向:

  CRISPR-Cas系统是广泛存在于原核生物中的一种由crRNA介导免疫系统,能够特异性识别并降解外源DNA或RNA。我们运用结构生物学方法,解析CRISPR-Cas系统中蛋白质-RNA/DNA复合体的三维空间结构,综合生物化学的研究手段,进一步探究CRISPR-Cas系统抵御外源核酸入侵的分子机理。

承担项目情况:

  1. 国家自然科学基金面上项目,III型CRISPR-Cas系统抗病毒的分子机制,2021.01-2024.12,在研,主持。

  2. 国家自然科学基金青年科学基金,anti-CRISPR蛋白对II型CRISPR-Cas系统抑制作用的分子机制研究,2018.01-2020.12,在研,主持。

代表论著:

  1. Huang X, Sun W, Cheng Z, Chen M, Li X, Wang J, Sheng G*, Gong W* and Wang Y*. Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2. Nature Communications, 2020.10, online.

  2. Sun W#, Yang J#, Cheng Z#, Amrani N, Liu C, Wang K, Ibraheim R, Edraki A, Huang X, Wang M, Wang J, Liu L, Sheng G, Yang Y, Lou J, Sontheimer E*, and Wang Y*. Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States. Molecular Cell, 2019, 76(6): 938-952.

  3. You L, Ma J, Wang J, Artamonova D, Wang M, Liu L, Xiang H, Severinov K, Zhang X*, Wang Y*. Structure Studies of the CRISPR-Csm Complex Reveal Mechanism of Co-transcriptional Interference. Cell 2019, 176, 239–253.

  4. Yin M, Wang J, Wang M, Li X, Zhang M, Wu Q*, Wang Y*. Molecular mechanism of directional CTCF recognition of a diverse range of genomic sites. Cell Res 2017, 27:1365-1377. 

  5. Liu L#, Li X#, Ma J#, Li Z#, You L, Wang J, Wang M, Zhang X*, Wang Y*. The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a. Cell 2017, 170:714-726 e710.

  6. Liu L, Li X, Wang J, Wang M, Chen P, Yin M, Li J, Sheng G, Wang Y*. Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities. Cell 2017, 168:121-134 e112.  

  7. Liu L, Chen P, Wang M, Li X, Wang J, Yin M, Wang Y*. C2c1-sgRNA Complex Structure Reveals RNA-Guided DNA Cleavage Mechanism. Mol Cell 2017, 65:310-322.

  8. Sheng G, Gogakos T, Wang J, Zhao H, Serganov A, Juranek S, Tuschl T, Patel DJ*, Wang Y*. Structure/cleavage-based insights into helical perturbations at bulge sites within T. thermophilus Argonaute silencing complexes. Nucleic Acids Res 2017, 45:9149-9163.

  9. Wang J#, Ma J#, Cheng Z, Meng X, You L, Wang M, Zhang X*, Wang Y*. A CRISPR evolutionary arms race: structural insights into viral anti-CRISPR/Cas responses. Cell Res 2016, 26:1165-1168. (#共同第一作者)

  10. Wang J#, Li J#, Zhao H, Sheng G, Wang M, Yin M, Wang Y*. Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems. Cell 2015, 163:840-853. (#共同第一作者)

  11. Zhao H, Sheng G, Wang J, Wang M, Bunkoczi G, Gong W, Wei Z, Wang Y*. Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Nature 2014, 515:147-150.

  12. Sheng G#, Zhao H#, Wang J, Rao Y, Tian W, Swarts DC, van der Oost J, Patel DJ*, Wang Y*. Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage. Proc Natl Acad Sci U S A 2014, 111:652-657.

 

(资料来源:王久宇副研究员,2020-11-16)