Genetically Encoded Phosphine Ligand for Metalloprotein Design
Hua-Zhen Duan, Cheng Hu, Yue-Lin Li, Shi-Hao Wang, Yan Xia, Xiaohong Liu*, Jiangyun Wang*, and Yong-Xiang Chen*
Abstract
Phosphine ligands are the most important class of ligands for cross-coupling reactions due to their unique electronic and steric properties. However, metalloproteins generally rely on nitrogen, sulfur, or oxygen ligands. Here, we report the genetic incorporation of P3BF, which contains a biocompatible borane-protected phosphine, into proteins. This step is followed by a straightforward one-pot strategy to perform deboronation and palladium coordination in aqueous and aerobic conditions. The genetically encoded phosphine ligand P3BF should significantly expand our ability to design functional metalloproteins.
附件下载：
Genetically Encoded Phosphine Ligand for Metalloprotein Design, J. Am. Chem. Soc, 23 Nov 2022
Journal Of The American Chemical Society, 23 November, 2022, DOI：https://doi.org/10.1021/jacs.2c09683
Genetically Encoded Phosphine Ligand for Metalloprotein Design
Hua-Zhen Duan, Cheng Hu, Yue-Lin Li, Shi-Hao Wang, Yan Xia, Xiaohong Liu*, Jiangyun Wang*, and Yong-Xiang Chen*
Abstract
Phosphine ligands are the most important class of ligands for cross-coupling reactions due to their unique electronic and steric properties. However, metalloproteins generally rely on nitrogen, sulfur, or oxygen ligands. Here, we report the genetic incorporation of P3BF, which contains a biocompatible borane-protected phosphine, into proteins. This step is followed by a straightforward one-pot strategy to perform deboronation and palladium coordination in aqueous and aerobic conditions. The genetically encoded phosphine ligand P3BF should significantly expand our ability to design functional metalloproteins.
文章链接：https://pubs.acs.org/doi/10.1021/jacs.2c09683
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