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Interplay between the bacterial protein deacetylase CobB and the second messenger c‐di‐GMP, EMBO J, 16 Aug 2019

发布时间:2019年08月16日

EMBO Journal, 16 August, 2019,DOI:https://doi.org/10.15252/embj.2018100948

Interplay between the bacterial protein deacetylase CobB and the second messenger c‐di‐GMP

Zhaowei Xu, Hainan Zhang, Xingrun Zhang, Hewei Jiang, Chengxi Liu, Fanlin Wu, Lili Qian, Bingbing Hao, Daniel M Czajkowsky, Shujuan Guo, Zhijing Xu, Lijun Bi, Shihua Wang, Haitao Li, Minjia Tan, Wei Yan, Lei Feng, Jingli Hou, Sheng‐ce Tao

Abstract

As a ubiquitous bacterial secondary messenger, c‐di‐GMP plays key regulatory roles in processes such as bacterial motility and transcription regulation. CobB is the Sir2 family protein deacetylase that controls energy metabolism, chemotaxis, and DNA supercoiling in many bacteria. Using an Escherichia coli proteome microarray, we found that c‐di‐GMP strongly binds to CobB. Further, protein deacetylation assays showed that c‐di‐GMP inhibits the activity of CobB and thereby modulates the biogenesis of acetyl‐CoA. Interestingly, we also found that one of the key enzymes directly involved in c‐di‐GMP production, DgcZ, is a substrate of CobB. Deacetylation of DgcZ by CobB enhances its activity and thus the production of c‐di‐GMP. Our work establishes a novel negative feedback loop linking c‐di‐GMP biogenesis and CobB‐mediated protein deacetylation.

文章链接:https://www.embopress.org/doi/full/10.15252/embj.2018100948

 

 

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