Super-resolution correlative light and electron microscopy (SR-CLEM) is a powerful approach for imaging specific molecules at the nanoscale in the context of the cellular ultrastructure. Epon epoxy resin embedding offers advantages for SR-CLEM, including ultrastructural preservation and high quality sectioning. However, Epon embedding eliminates fluorescence from most fluorescent proteins. We describe a photocontrollable fluorescent protein, mEosEM, that can survive Epon embedding after osmium tetroxide (OsO4) treatment for improved SR-CLEM.
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mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM,Nat Methods, 14 Oct 2019
Nature Methods, 14 October, 2019，DOI：https://doi.org/10.1038/s41592-019-0613-6
mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM
Zhifei Fu, Dingming Peng, Mingshu Zhang, Fudong Xue, Rui Zhang, Wenting He, Tao Xu & Pingyong Xu
Abstract
Super-resolution correlative light and electron microscopy (SR-CLEM) is a powerful approach for imaging specific molecules at the nanoscale in the context of the cellular ultrastructure. Epon epoxy resin embedding offers advantages for SR-CLEM, including ultrastructural preservation and high quality sectioning. However, Epon embedding eliminates fluorescence from most fluorescent proteins. We describe a photocontrollable fluorescent protein, mEosEM, that can survive Epon embedding after osmium tetroxide (OsO4) treatment for improved SR-CLEM.
文章链接：https://www.nature.com/articles/s41592-019-0613-6
相关报道：http://www.ibp.cas.cn/kyjz/zxdt/201910/t20191014_5407533.html