Nanoscale magnetic imaging of ferritins in a single cell
Pengfei Wang, Sanyou Chen, Maosen Guo, Shijie Peng, Mengqi Wang, Ming Chen, Wenchao Ma, Rui Zhang, Jihu Su, Xing Rong, Fazhan Shi, Tao Xu, and Jiangfeng Du
Abstract
The in situ measurement of the distribution of biomolecules inside a cell is one of the important goals in life science. Among various imaging techniques, magnetic imaging (MI) based on the nitrogen-vacancy (NV) center in diamond provides a powerful tool for the biomolecular research, while the nanometer-scale MI of intracellular proteins remains a challenge. Here, we use ferritin as a demonstration to realize the MI of endogenous proteins in a single cell using the NV center as the sensor. With the scanning, intracellular ferritins are imaged with a spatial resolution of ca. 10 nm, and ferritin-containing organelles are colocalized by correlative MI and electron microscopy. The approach paves the way for nanoscale MI of intracellular proteins.
附件下载：
Nanoscale magnetic imaging of ferritins in a single cell, Science Advances, 10 Apr 2019
Science Advances, 10 , 2019， DOI: http://dx.doi.org/10.1126/sciadv.aau8038
Nanoscale magnetic imaging of ferritins in a single cell
Pengfei Wang, Sanyou Chen, Maosen Guo, Shijie Peng, Mengqi Wang, Ming Chen, Wenchao Ma, Rui Zhang, Jihu Su, Xing Rong, Fazhan Shi, Tao Xu, and Jiangfeng Du
Abstract
The in situ measurement of the distribution of biomolecules inside a cell is one of the important goals in life science. Among various imaging techniques, magnetic imaging (MI) based on the nitrogen-vacancy (NV) center in diamond provides a powerful tool for the biomolecular research, while the nanometer-scale MI of intracellular proteins remains a challenge. Here, we use ferritin as a demonstration to realize the MI of endogenous proteins in a single cell using the NV center as the sensor. With the scanning, intracellular ferritins are imaged with a spatial resolution of ca. 10 nm, and ferritin-containing organelles are colocalized by correlative MI and electron microscopy. The approach paves the way for nanoscale MI of intracellular proteins.
文章链接：https://advances.sciencemag.org/content/5/4/eaau8038