CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development, Cell Research advance online publication 6 February 2015
发布时间：2015年02月15日
Cell Research, advance online publication 6 February 2015; doi: 10.1038/cr.2015.15
CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development
Tao Tu1, Chunxia Zhang2, Huiwen Yan1, Yongting Luo1, Ruirui Kong3, Pushuai Wen3, Zhongde Ye1, Jianan Chen1, Jing Feng1, Feng Liu2, Jane Y Wu3,4 and Xiyun Yan1
1Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
2State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
3State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
4Department of Neurology, Center for Genetic Medicine, Lurie Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA
Received 15 December 2014; Revised 4 January 2015; Accepted 5 January 2015
Advance online publication 6 February 2015
Abstract
Angiogenesis, a process that newly-formed blood vessels sprout from pre-existing ones, is vital for vertebrate development and adult homeostasis. Previous studies have demonstrated that the neuronal guidance molecule netrin-1 participates in angiogenesis and morphogenesis of the vascular system. Netrin-1 exhibits dual activities in angiogenesis: either promoting or inhibiting angiogenesis. The anti-angiogenic activity of netrin-1 is mediated by UNC5B receptor. However, how netrin-1 promotes angiogenesis remained unclear. Here we report that CD146, an endothelial transmembrane protein of the immunoglobulin superfamily, is a receptor for netrin-1. Netrin-1 binds to CD146 with high affinity, inducing endothelial cell activation and downstream signaling in a CD146-dependent manner. Conditional knockout of the cd146 gene in the murine endothelium or disruption of netrin-CD146 interaction by a specific anti-CD146 antibody blocks or reduces netrin-1-induced angiogenesis. In zebrafish embryos, downregulating either netrin-1a or CD146 results in vascular defects with striking similarity. Moreover, knocking down CD146 blocks ectopic vascular sprouting induced by netrin-1 overexpression. Together, our data uncover CD146 as a previously unknown receptor for netrin-1 and also reveal a functional ligand for CD146 in angiogenesis, demonstrating the involvement of netrin-CD146 signaling in angiogenesis during vertebrate development.
附件下载：
CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development, Cell Research advance online publication 6 February 2015
Cell Research, advance online publication 6 February 2015; doi: 10.1038/cr.2015.15
CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development
Tao Tu1, Chunxia Zhang2, Huiwen Yan1, Yongting Luo1, Ruirui Kong3, Pushuai Wen3, Zhongde Ye1, Jianan Chen1, Jing Feng1, Feng Liu2, Jane Y Wu3,4 and Xiyun Yan1
1Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
2State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
3State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
4Department of Neurology, Center for Genetic Medicine, Lurie Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA
Correspondence: Xiyun Yan, E-mail: yanxy@ibp.ac.cn; Jane Y Wu, E-mail: jane-wu@northwestern.edu
Received 15 December 2014; Revised 4 January 2015; Accepted 5 January 2015
Advance online publication 6 February 2015
Abstract
Angiogenesis, a process that newly-formed blood vessels sprout from pre-existing ones, is vital for vertebrate development and adult homeostasis. Previous studies have demonstrated that the neuronal guidance molecule netrin-1 participates in angiogenesis and morphogenesis of the vascular system. Netrin-1 exhibits dual activities in angiogenesis: either promoting or inhibiting angiogenesis. The anti-angiogenic activity of netrin-1 is mediated by UNC5B receptor. However, how netrin-1 promotes angiogenesis remained unclear. Here we report that CD146, an endothelial transmembrane protein of the immunoglobulin superfamily, is a receptor for netrin-1. Netrin-1 binds to CD146 with high affinity, inducing endothelial cell activation and downstream signaling in a CD146-dependent manner. Conditional knockout of the cd146 gene in the murine endothelium or disruption of netrin-CD146 interaction by a specific anti-CD146 antibody blocks or reduces netrin-1-induced angiogenesis. In zebrafish embryos, downregulating either netrin-1a or CD146 results in vascular defects with striking similarity. Moreover, knocking down CD146 blocks ectopic vascular sprouting induced by netrin-1 overexpression. Together, our data uncover CD146 as a previously unknown receptor for netrin-1 and also reveal a functional ligand for CD146 in angiogenesis, demonstrating the involvement of netrin-CD146 signaling in angiogenesis during vertebrate development.
相关报道：http://www.ibp.cas.cn/kyjz/zxdt/201502/t20150213_4313869.html
文章链接：http://www.nature.com/cr/journal/vaop/ncurrent/full/cr201515a.html