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Significant Expansion of Fluorescent Protein Sensing Ability ... Superior Photo-Induced Electron-Transfer Quenchers,JACS(J. Am. Chem. Soc.), September 8, 2014

发布时间:2014年09月11日

Journal of the American Chemical SocietyPublication Date (Web): September 8, 2014,DOI: 10.1021/ja505219r

Significant Expansion of Fluorescent Protein Sensing Ability through the Genetic Incorporation of Superior Photo-Induced Electron-Transfer Quenchers

Xiaohong Liu ?, Li Jiang ?, Jiasong Li ?, Li Wang ?, Yang Yu ?, Qing Zhou ?, Xiaoxuan Lv ?, Weimin Gong ?, Yi Lu §, and Jiangyun Wang *?

Abstract

Photo-induced electron transfer (PET) is ubiquitous for photosynthesis and fluorescent sensor design. However, genetically coded PET sensors are underdeveloped, due to the lack of methods to site-specifically install PET probes on proteins. Here we describe a family of acid and Mn(III) turn-on fluorescent protein (FP) sensors, named iLovU, based on PET and the genetic incorporation of superior PET quenchers in the fluorescent flavoprotein iLov. Using the iLovU PET sensors, we monitored the cytoplasmic acidification process, and achieved Mn(III) fluorescence sensing for the first time. The iLovU sensors should be applicable for studying pH changes in living cells, monitoring biogentic Mn(III) in the environment, and screening for efficient manganese peroxidase, which is highly desirable for lignin degradation and biomass conversion. Our work establishes a platform for many more protein PET sensors, facilitates the de novo design of metalloenzymes harboring redox active residues, and expands our ability to probe protein conformational dynamics.

相关报道:http://www.ibp.cas.cn/kyjz/zxdt/201409/t20140911_4200714.html

文章链接:http://pubs.acs.org/doi/abs/10.1021/ja505219r

 

 

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