Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
Dongjie Zhu, Xiangxi Wang, Qianglin Fang, James L Van Etten, Michael G Rossmann, Zihe Rao & Xinzheng Zhang
Abstract
The Ewald sphere effect is generally neglected when using the Central Projection Theorem for cryo electron microscopy single-particle reconstructions. This can reduce the resolution of a reconstruction. Here we estimate the attainable resolution and report a “block-based” reconstruction method for extending the resolution limit. We find the Ewald sphere effect limits the resolution of large objects, especially large viruses. After processing two real datasets of large viruses, we show that our procedure can extend the resolution for both datasets and can accommodate the flexibility associated with large protein complexes.
附件下载：
Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions, Nat Commun, 9：1552, 19 Apr 2018
Nature Communications, 19 Apr 2018，DOI: 10.1038/s41467-018-04051-9
Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
Dongjie Zhu, Xiangxi Wang, Qianglin Fang, James L Van Etten, Michael G Rossmann, Zihe Rao & Xinzheng Zhang
Abstract
The Ewald sphere effect is generally neglected when using the Central Projection Theorem for cryo electron microscopy single-particle reconstructions. This can reduce the resolution of a reconstruction. Here we estimate the attainable resolution and report a “block-based” reconstruction method for extending the resolution limit. We find the Ewald sphere effect limits the resolution of large objects, especially large viruses. After processing two real datasets of large viruses, we show that our procedure can extend the resolution for both datasets and can accommodate the flexibility associated with large protein complexes.
文章链接：https://www.nature.com/articles/s41467-018-04051-9
相关报道：http://www.ibp.cas.cn/kyjz/zxdt/201804/t20180423_5000633.html