1Key Laboratory of Infection and Immunity of CAS, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
2Department of Laboratory AnimalScience, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China.
3CAS Center for Excellence in Biomacromolecules, ChineseAcademy of Sciences, Beijing, China.
4These authors contributed equally to this work. Correspondence should be addressed to Z.L. (zhihualiu@ibp.ac.cn) orH. Wei (weihong63528@163.com).
Abstract
Mucosal immunity protects a host from intestinal inflammation and infection and is profoundly influenced by symbiotic bacteria. Zhihua Liu lab at the Institute of Biophysics, Chinese Academy of Sciences reported that in mice symbiotic bacteria directed selective cargo sorting in Paneth cells to promote symbiosis through Nod2, a cytosolic bacterial sensor, and the multifunctional protein kinase LRRK2, both encoded by inflammatory bowel disease (IBD)-associated genes. Commensals recruited Nod2 onto lysozyme-containing dense core vesicles (DCVs), which was required for DCV localization of LRRK2 and a small GTPase, Rab2a. Deficiency of Nod2, LRRK2 or Rab2a or depletion of commensals resulted in lysosomal degradation of lysozyme. Thus, commensal bacteria and host factors orchestrate the lysozyme-sorting process to protect the host from enteric infection, implicating Paneth cell dysfunction in IBD pathogenesis.
附件下载：
Commensal bacteria direct selective cargo sorting to promote symbiosis，Nature Immunology, Published online 3 August 2015
Nature Immunology, Published online 3 August 2015, DOI: doi:10.1038/ni.3233
Commensal bacteria direct selective cargo sorting to promote symbiosis
Qin Zhang1,4, Ying Pan1,4, Ruiqing Yan1, Benhua Zeng2, Haifang Wang1, Xinwen Zhang1, Wenxia Li2,Hong Wei2 &Zhihua Liu1,3
1Key Laboratory of Infection and Immunity of CAS, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
2Department of Laboratory AnimalScience, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China.
3CAS Center for Excellence in Biomacromolecules, ChineseAcademy of Sciences, Beijing, China.
4These authors contributed equally to this work. Correspondence should be addressed to Z.L. (zhihualiu@ibp.ac.cn) orH. Wei (weihong63528@163.com).
Abstract
Mucosal immunity protects a host from intestinal inflammation and infection and is profoundly influenced by symbiotic bacteria. Zhihua Liu lab at the Institute of Biophysics, Chinese Academy of Sciences reported that in mice symbiotic bacteria directed selective cargo sorting in Paneth cells to promote symbiosis through Nod2, a cytosolic bacterial sensor, and the multifunctional protein kinase LRRK2, both encoded by inflammatory bowel disease (IBD)-associated genes. Commensals recruited Nod2 onto lysozyme-containing dense core vesicles (DCVs), which was required for DCV localization of LRRK2 and a small GTPase, Rab2a. Deficiency of Nod2, LRRK2 or Rab2a or depletion of commensals resulted in lysosomal degradation of lysozyme. Thus, commensal bacteria and host factors orchestrate the lysozyme-sorting process to protect the host from enteric infection, implicating Paneth cell dysfunction in IBD pathogenesis.
文章链接：http://www.nature.com/ni/journal/vaop/ncurrent/full/ni.3233.html
相关报道：http://www.ibp.cas.cn/kyjz/zxdt/201508/t20150818_4412265.html