ACovalent Approach for Site-Specific RNA Labeling in Mammalian Cells，Angew. Chem. Int. Ed. 2015, 54, 4597–4602

Angewandte Chemie International Edition 2015, 54, 4597–4602，DOI: 10.1002/anie.201410433

A Covalent Approach for Site-Specific RNA Labeling in Mammalian Cells

Fahui Li, Jianshu Dong, Xiaosong Hu, Weimin Gong, Jiasong Li, Jing Shen, Huifang Tian, and Jiangyun Wang*

Abstract

Advances in RNA research and RNA nanotechnology depend on the ability to manipulate and probe RNAwith high precision through chemical approaches, both in vitro and in mammalian cells. However, covalent RNA labeling methods with scope and versatility comparable to those of current protein labeling strategies are underdeveloped. A method is reported for the site- and sequence-specific covalent labeling of RNAs in mammalian cells by using tRNAIle2-agmatidine synthetase (Tias) and click chemistry. The crystal structure of Tias in complex with an azide-bearing agmatine analogue was solved to unravel the structural basis for Tias/substrate recognition. The unique RNA sequence specificity and plastic Tias/substrate recognition enable the site-specific transfer of azide/alkyne groups to an RNA molecule of interest in vitro and in mammalian cells. Subsequent click chemistry reactions facilitate the versatile labeling, functionalization, and visualization of target RNA.

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文章链接：http://onlinelibrary.wiley.com/doi/10.1002/anie.201410433/pdf