Conditional targeted genome editing using somatically expressed TALENs in C. elegans ，Nature Biotechnology， Published online 18 August 2013

Nature Biotechnology, Published online 18 August 2013, doi:10.1038/nbt.2674

Conditional targeted genome editing using somatically expressed TALENs in C. elegans

Ze Cheng,Peishan Yi,Xiangming Wang,Yongping Chai,Guoxin Feng, Yihong Yang,Xing Liang,Zhiwen Zhu, Wei Li & Guangshuo Ou

Abstract

We have developed a method for the generation of conditional knockouts in Caenorhabditis elegans by expressing transcription activator–like effector nucleases (TALENs) in somatic cells. Using germline transformation with plasmids encoding TALENs under the control of an inducible or tissue-specific promoter, we observed effective gene modifications and resulting phenotypes in specific developmental stages and tissues. We further used this method to bypass the embryonic requirement of cor-1, which encodes the homolog of human severe combined immunodeficiency (SCID) protein coronin, and we determined its essential role in cell migration in larval Q-cell lineages. Our results show that TALENs expressed in the somatic cells of model organisms provide a versatile tool for functional genomics.

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文章链接：http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.2674.html