Expanding the Genetic Code for Photoclick Chemistry in E.coli, Mammalian Cells, and A.thalian. Angewandte Chemie International Edition，first published online: 19 JUL 2013

发布时间：2013-07-22

Angewandte Chemie International Edition，Article first published online: 19 JUL 2013， DOI: 10.1002/anie.201303477

Expanding the Genetic Code for Photoclick Chemistry in E. coli, Mammalian Cells, and A. thalian

Fahui Li, Hua Zhang, Yun Sun, Yanchao Pan, Juanzuo Zhou, Prof. Dr. Jiangyun Wang*

Abstract

Breaking the (genetic) code: N-ε-acryl- lysine was genetically incorporated into proteins in bacterial cells, mammalian cells, and Arabidopsis thaliana by co-expressing a pyrrolysyl-tRNA mutant synthetase and its cognate tRNA in the model organism. A photoclick reaction driven by irradiation by 365 nm UV light or 405 nm laser light and allowed for site-specific protein labeling within minutes both in vitro and in vivo.

相关报道：http://www.ibp.cas.cn/kyjz/zxdt/201307/t20130722_3903406.html

全文链接：http://onlinelibrary.wiley.com/doi/10.1002/anie.201303477/abstract

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