A Genetically Encoded 19F NMR Probe for Tyrosine Phosphorylation，Angewandte Chemie International Edition，Article first published online: 28 FEB 2013

Angewandte Chemie International Edition，Article first published online: 28 FEB 2013，DOI: 10.1002/anie.201300463

A Genetically Encoded 19F NMR Probe for Tyrosine Phosphorylation

Fahui Li2, Pan Shi1,4, Jiasong Li2, Fan Yang3, Tianyuan Wang2, Wei Zhang2, Feng Gao2, Wei Ding2, Dong Li1, Juan Li1, Ying Xiong1, Jinpeng Sun3, Weimin Gong2,*, Changlin Tian1,4,*, Jiangyun Wang2,*

Simple and selective:

Tyrosine phosphorylation is a pivotal post-translational modification which regulates the enzymatic activity, protein conformation, and protein–protein interactions. The highly efficient genetic incorporation of 3,5-difluorotyrosine (F2Y) in E. coli and the use of F2Y as a 19F NMR probe for the tyrosine phosphorylation are reported (see picture).

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全文链接：http://onlinelibrary.wiley.com/doi/10.1002/anie.201300463/abstract