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胡俊杰  博士 研究员 博士生导师  

中科院生物物理所,生物大分子国家重点实验室,研究组长

研究方向:内质网形态与功能

电子邮件:huj@ibp.ac.cn

电       话:010-64886852

通讯地址:北京市朝阳区大屯路15号(100101)

英文版个人网页:http://english.ibp.cas.cn/faculty/index_18316.html?json=http://www.ibp.cas.cn/sourcedb_ibp_cas/cn/ibpexport/EN_xsszmH_K/202005/t20200519_5583007.json

简       历:

  2000年毕业于复旦大学生命科学学院生物化学系。随后,赴美国密歇根大学生物学系攻读博士学位。

  2001年转学至纽约大学医学院,学习研究胰岛素信号转导的结构基础。

  2005年获药理学系博士学位。

  2005年12月至2008年6月,于哈佛医学院细胞生物学系/霍华德休斯医学研究所从事博士后工作,研究管状内质网成形的机制。

  2008-2012年担任南开大学生命科学学院教授。

  2013年起担任中国科学院生物物理研究所研究员。先后获得霍华德.休斯医学研究所(HHMI)国际青年科学家奖、国家自然科学基金委杰出青年科学基金,入选中组部“青年拔尖人才”、“科技创新领军人才”以及教育部“长江学者奖励计划”青年项目。

获奖及荣誉:

社会任职:

研究方向:

  本实验室主要研究内质网等重要细胞器的形态与功能。内质网是一个广泛连续的膜网络,承担了蛋白合成、脂类合成和钙存储等重要功能。内质网的形态主要包括片状和管状,然而这些不同形态的膜结构如何在同一膜系统内形成,我们还知之甚少。前期研究发现一组膜蛋白reticulons和DP1/Yop1p参与了管状内质网的成形,这些膜蛋白只在管状内质网中存在,他们的过表达和敲除都会影响内质网的形态。我们纯化了两个成管膜蛋白,并发现他们在体外与脂肪重组后能形成管状膜结构。我们最近又发现嵌膜GTP酶atlastin等参与了内质网管状网络的形成,这些蛋白的缺失或突变导致内质网分叉减少,也能引起遗传性痉挛性截瘫。我们还通过结构解析等实验手段,剖析了atlastin介导膜融合的可能机制。总体来说,我们将采用细胞生物学,生物化学及结构生物学等多种方法深入研究内质网塑形和重装的分子机制,内质网的形态与功能的关联,以及与内质网功能相关的疾病。

承担项目情况:

代表论著:

  1. Wang M, Guo X, Yang X, Zhang B, Ren J, Liu A, Ran Y, Yan B, Chen F, Guddat LW, Hu J, Li J, Rao Z. Mycobacterial dynamin-like protein IniA mediates membrane fission. Nat Commun. 2019 Aug 29

  2. Niu L, Ma T, Yang F, Yan B, Tang X, Yin H, Wu Q, Huang Y, Yao ZP, Wang J, Guo Y, Hu J. Atlastin-mediated membrane tethering is critical for cargo mobility and exit from the endoplasmic reticulum. Proc Natl Acad Sci U S A. 2019 Jul 9.

  3. Zhou X, He Y, Huang X, Guo Y, Li D, Hu J. Reciprocal regulation between lunapark and atlastin facilitates ER three-way junction formation. Protein Cell. 2019 Jul.

  4. Gao A, Sun T, Ma G, Cao J, Hu Q, Chen L, Wang Y, Wang Q, Sun J, Wu R, Wu Q, Zhou J, Liu L, Hu J, Dong JT, Zhu Z. LEM4 confers tamoxifen resistance to breast cancer cells by activating cyclin D-CDK4/6-Rb and ERα pathway. Nat Commun. 2018 Oct 9

  5. Wu Y, Li X, Jia J, Zhang Y, Li J, Zhu Z, Wang H, Tang J, Hu J. Transmembrane E3 ligase RNF183 mediates ER stress-induced apoptosis by degrading Bcl-xL. Proc Natl Acad Sci USA. 2018 Mar 5.

  6. Yan L, Qi Y, Huang X, Yu C, Lan L, Guo X, Rao Z, Hu J, Lou Z. Structural basis for GTP hydrolysis and conformational change of MFN1 in mediating membrane fusion. Nature Structural & Molecular Biology. 2018 Feb 26.

  7. Zhu Y, Zhang G, Lin S, Shi J, Zhang H, Hu J. Sec61β facilitates the maintenance of endoplasmic reticulum homeostasis by associating microtubules. Protein & Cell. 2017 Nov 22

  8. Huang X, Zhou X, Hu X, Joshi AS, Guo X, Zhu Y, Chen Q, Prinz WA, Hu J. Sequences flanking the transmembrane segments facilitate mitochondrial localization and membrane fusion by mitofusin. Proc Natl Acad Sci USA. 2017 Nov 14; 114(46): E9863-E9872

  9. Wang X, Li S, Wang H, Shui W, Hu J. Quantitative proteomics reveal proteins enriched in tubular endoplasmic reticulum of Saccharomyces cerevisiae. eLife. 2017 Mar 13, 6

  10. Li J, Yan B, Si H, Peng X, Zhang SL, Hu J. Atlastin regulates store-operated calcium entry for nerve growth factor-induced neurite outgrowth. Scientific reports. 2017 Feb 27, 7:43490

  11. Qi Y, Yan L, Yu C, Guo X, Zhou X, Hu X, Huang X, Rao Z, Lou Z, Hu J. Structures of human mitofusin 1 provide insight into mitochondrial tethering.  J Cell Biol. 2016 Dec 5; 215(5): 621-629.

  12. Zhang H, Hu J. Shaping the Endoplasmic Reticulum into a Social Network. Trends in cell biology. 2016, 26(12): 934~943.

  13. Hu J, Rapoport TA. Fusion of the endoplasmic reticulum by membrane-bound GTPases. Semin Cell Dev Biol. 2016. Dec; 60: 105-111.

  14. Yan L, Sun S, Wang W, Shi J, Hu X, Wang S, Su D, Rao Z, Hu J, Lou Z. Structures of the yeast dynamin-like GTPase Sey1p provide insight into homotypic ER fusion. J Cell Biol. 2015 Sep 14;210(6):961-72.

  15. Liu TY, Bian X, Romano FB, Shemesh T, Rapoport TA, Hu J.  Cis and trans interactions between atlastin molecules during membrane fusion. Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):E1851-60.

  16. Zhang M, Wu F, Shi J, Zhu Y, Zhu Z, Gong Q, Hu J.  ROOT HAIR DEFECTIVE3 family of dynamin-like GTPases mediates homotypic endoplasmic reticulum fusion and is essential for Arabidopsis development. Plant Physiol. 2013 Oct;163(2):713-20.

  17. Feng Y , Yu W, Li X, Lin S, Zhou Y, Hu J, Liu X.  Structural insight into Golgi membrane stacking by GRASP65 and GRASP55 proteins  J Biol Chem. 2013 Sep 27;288(39):28418-27.

  18. Liu TY, Bian X, Sun S, Hu X, Klemm RW, Prinz WA, Rapoport TA, Hu J. Lipid interaction of the C terminus and association of the transmembrane segments facilitate atlastin-mediated homotypic endoplasmic reticulum fusion. Proc Natl Acad Sci U S A. 2012 Aug 7;109(32):E2146-54.

  19. Hu J, Prinz WA, Rapoport TA. Weaving the web of ER tubules. Cell. 2011 Dec 9;147(6):1226-31. Review.

  20. Bian X, Klemm RW, Liu TY, Zhang M, Sun S, Sui X, Liu X, Rapoport TA, Hu J. Structures of the Atlastin GTPase provide Insight into homotypic Fusion of ER membranes. Proc Natl Acad Sci U S A. 2011 108, Mar 8;108(10):3976-81.

  21. Hu J, Shibata Y, Zhu PP, Voss C, Rismanchi N, Prinz WA, Rapoport TA, Blackstone C. A class of Dynamin-like GTPases involved in the generation of the tubular ER network. Cell. 2009 Aug 7;138(3):549-61.

  22. Hu J, Shibata Y, Voss C, Shemesh T, Li Z, Coughlin M, Kozlov MM, Rapoport TA, and Prinz WA. Membrane Proteins of the Endoplasmic Reticulum Induce High-curvature Tubules. Science. 2008 Feb 29;319(5867):1247-50.

  23. Hu J, Liu J, Ghirlando R, Saltiel AR, Hubbard SR. Structural Basis for the Recruitment of the Adaptor Protein APS to the Activated Insulin Receptor. Mol Cell. 2003 Dec 1;12(6):1379-89.

  

(资料来源:胡俊杰研究员,2019-11-25)