网站地图 联系我们搜索内部网English | 中国科学院
首页 所况简介 机构设置 科研队伍 科学研究平台 院地合作 党群园地 国际交流 博士后 研究生教育 科学传播 信息公开
科研进展
成果报道
最新重要论文(影响因子PNAS及以上)
发表论文数据库
所级学术报告
科学成果
发表论文
专著
专利
获奖
专题
所史丛书
所庆专辑
建所50周年画册
现在位置:首页 > 科研进展 > 最新重要论文(影响因子PNAS及以上)
FOXO3-Engineered Human ESC-Derived Vascular Cells Promote Vascular Protection and Regeneration, Cell Stem Cell, 17 Jan 2019
2019-01-17 | 【     】【打印】【关闭

Cell Stem Cell, 17 January, 2019, DOI: https://doi.org/10.1016/j.stem.2018.12.002

FOXO3-Engineered Human ESC-Derived Vascular Cells Promote Vascular Protection and Regeneration

Pengze Yan, Qingqing Li, Lixia Wang, Ping Lu, Keiichiro Suzuki, Zunpeng Liu, Jinghui Lei, Wei Li, Xiaojuan He, Si Wang, Jianjian Ding, Piu Chan, Weiqi Zhang, Moshi Song, Juan Carlos Izpisua Belmonte, Jing Qu, Fuchou Tang, Guang-Hui Liu

Summary

FOXO3 is an evolutionarily conserved transcription factor that has been linked to longevity. Here we wanted to find out whether human vascular cells could be functionally enhanced by engineering them to express an activated form of FOXO3. This was accomplished via genome editing at two nucleotides in human embryonic stem cells, followed by differentiation into a range of vascular cell types. FOXO3-activated vascular cells exhibited delayed aging and increased resistance to oxidative injury compared with wild-type cells. When tested in a therapeutic context, FOXO3-enhanced vascular cells promoted vascular regeneration in a mouse model of ischemic injury and were resistant to tumorigenic transformation both in vitro and in vivo. Mechanistically, constitutively active FOXO3 conferred cytoprotection by transcriptionally downregulating CSRP1. Taken together, our findings provide mechanistic insights into FOXO3-mediated vascular protection and indicate that FOXO3 activation may provide a means for generating more effective and safe biomaterials for cell replacement therapies.

文章链接:https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(18)30592-7

相关报道:http://www.ibp.cas.cn/kyjz/zxdt/201901/t20190118_5231450.html

 

评 论
                 
版权所有:中国科学院生物物理研究所     京ICP备05002792号 京公网安备 110402500011 号
地址:北京市朝阳区大屯路15号(100101) 电话:010-64889872
意见反馈联系人:侯文茹 电子邮件:houwenru@ibp.ac.cn