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  张先恩 博士 研究员 博士生导师

  中科院生物物理所,生物大分子国家重点实验室,研究组长

  研究方向:生物传感,纳米生物学和细胞分子影像 

  电子邮件(E-mail):zhangxe(AT)ibp.ac.cn

  电话(Tel):010-64888148

  邮政编码:100101

  英文版个人网页 

  简历 & 研究组工作摘要

  2013年5月至今,在中科院生物物理所生物大分子国家重点实验室担任PI研究员、中科院生物大分子科教融合卓越中心特聘研究员。

  1982年毕业于湖北大学生物系,1985年在中科院武汉病毒所获硕士学位(微生物学专业),1995年在中科院微生物所获博士学位(在职,生物化学专业),2015年获加拿大Alberta大学荣誉科学博士学位。

  1993年在中科院武汉病毒所晋升研究员。1992年起历任中科院武汉病毒所、中科院武汉分院和国家科学技术部基础研究司领导职务。曾先后在加拿大、美国和英国相关学术机构从事合作研究。

  主要学术兼职:中国生物工程学会副理事长,英国皇家化学会会士(RSC Fellow),美国医学与生物工程院会士(AIMBE Fellow), 亚洲生物技术协会(AFOB)纳米生物技术/生物传感/生物芯片分会共同创始会长,世界生物传感学术大会常设组委会委员;《生物工程学报》任执行主编,Biosensors & Bioelectronics、Science China-Life Science、《科学通报》、《中科院院刊》、Advanced Biosystems、《合成生物学》、《高等学校化学学报》等编委或顾问;亚太经合组织(APEC)首席科学顾问及相当机构(CSAE)会议中国代表。国家973计划专家顾问组副组长,科技部生物技术中心专家委员会成员,曾担任中国微生物学会副理事长、中国生物物理学会副理事长和伦敦纳米中心顾问小组成员。

  曾获中科院首届青年科学家奖(1989)、中科院有突出贡献中青年专家(1994)、湖北省自然科学一等奖(2015)等荣誉。

  发表科学论文260余篇(其中SCI收录220余篇),生物传感器和生物芯片相关专著3部。

  主要研究方向:生物传感、纳米生物学和细胞分子影像,并用其阐述病毒学和肿瘤学科学问题。

  主要工作和进展:

  1) 纳米生物学:利用病毒、细菌等天然生物大分子的特异性识别功能和自组装性能,通过理性设计,构建一维、二维和三维以及多层级的生物纳米载荷体系,构建高灵敏度生物传感系统和新型生物载药体系。目前的研究重点是发展组合抗原纳米疫苗。

  2) 细胞分子影像:已经创建了系列红色、远红和近红外荧光蛋白分子传感系统(BiFC,TriFC等)和“彩色病毒”。目前的研究重点是活细胞中单颗粒病毒示踪及与宿主细胞互作的可视化,活细胞染色质单拷贝基因精准标识和几何空间定位,肿瘤细胞表面抗原的超分辨精准表征(种类、定位、分布、定量等)。

  3) 生物传感:利用生物元件的特异性识别原理,结合物理和化学手段,建立了系列生物传感模式,包括微生物细胞电极、多功能酶电极、基因光学传感、免疫表面声波传感、结核分枝杆菌全蛋白组芯片等。目前的研究重点是穿戴式生化指标酶电极、纳米生物电极、细胞生物传感器。快速耐药检测微生物电极等。

  代表性论文

  一、通讯作者论文(近五年发表)

  1. Lin Huang, Ning Gu, Xian-En Zhang*, Dian-Bing Wang* (2019). Light-Inducible Exosome-Based Vehicle for Endogenous RNA Loading and Delivery to Leukemia Cells. Advanced Functional Materials, 29(9):1807189.

  2. Yan Xi, Dian-Bing Wang, Tingting Wang, Lin Huang, Xian-En Zhang* (2019). Quantitative Profiling of CD13 on Single Acute Myeloid Leukemia Cells by Super-resolution imaging and its Implication in Targeted Drug Susceptibility Assessment. Nanoscale, 11(4):1737-1744.

  3. Dongge Guo, Dian-Bing Wang, Chong Liu, Song Lu, Yu Hou, Xian-En Zhang* (2019). CRISPR-based genomic loci labeling revealed ordered spatial organization of chromatin in living diploid human cells. BBA-Molecular Cell Research, 1866(12):118518.

  4. Lingling Li, Chengchen Xu, Wenjing Zhang, Francesco Secundo, Chunyan Li, Zhi-Ping Zhang, Xian-En Zhang*, Feng Li* (2019). Cargo-Compatible Encapsulation in Virus-Based Nanoparticles. Nano Letters, 19(4):2700-2706.

  5. Tingting Wang, Guang Li, Dianbing Wang, Feng Li, Dong Men, Tao Hu, Yan Xi, and Xian-En Zhang* (2019). Quantitative profiling of integrin αvβ3 on single cells with quantum dot labeling to reveal the phenotypic heterogeneity of glioblastoma. Nanoscale, 11(39):18224-18231.

  6. Yiwei Li, Juan Zhou, Jin Song, Xiaosheng Liang, Zhiping Zhang, Dong Men*, Dianbing Wang, Xian-En Zhang* (2019). Chemical Nature of Electrochemical activation of Carbon Electrodes. Biosensors & Bioelectronics, 144: 111534.

  7. Sha Wei, Dianbing Wang, Hua Li, Lijun Bi, Jiaoyu Deng, Guofeng Zhu, Jibin Zhang, Chuanyou Li, Min Li, Yuan Fang, Guimin Zhang, Jian Chen, Shengce Tao, Xian-En Zhang* (2019). Fatty acylCoA synthetase FadD13 regulates proinflammatory cytokine secretion dependent on the NF‐κB signalling pathway by binding to eEF1A1. Cellular Microbiology, e13090.

  8. Yuanyuan Chen, Zhengyan Zhan, Hongtai Zhang, Lijun Bi, Xian-En Zhang*, Yu Vincent Fu* (2019). Kinetic analysis of DNA compaction by mycobacterial integration host factor at the single-molecule level. Tuberculosis, 119, 101862.

  9. 黄琳,王殿冰,顾宁,张先恩* (2019). 高效装载细胞内源蛋白工程化外泌体的构建. 生物工程学报,(8):1537-1545.

  10. Xian-En Zhang* (2018). Forty years of highly synchronous development in China’s scientific research and economy. National Science Review, 5 (6): 796–798.

  11. Fan Yang, Xiaolei Zuo, Chunhai Fan*, Xian-En Zhang* (2018). Biomacromolecular nanostructures-based interfacial engineering: from precise assembly to precision biosensing. National Science Review, 5 (5): 740–755.

  12. Lin Huang, Dian-Bing Wang, Netrapal Singh, Fang Yang, Ning Gu*, Xian-En Zhang* (2018). A dual-signal amplification platform for sensitive fluorescence biosensing of leukemia-derived exosomes. Nanoscale, 10(43): 20289-20295.

  13. Ye Yuan, Dian-Bing Wang, Jibin Zhang, Ji Liu, Jian Chen, Xian-En Zhang* (2018). Raman spectra of the GFP-like fluorescent proteins. Biophysics reports, 4(5): 265-272.

  14. Jigang Wang, Ti Fang, Ming Li, Wenjing Zhang, Zhi-Ping Zhang, Xian-En Zhang*, Feng Li* (2018). Intracellular delivery of peptide drugs using viral nanoparticles of bacteriophage P22: covalent loading and cleavable release. Journal of Materials Chemistry B, 6(22):3716-3726.

  15. Wen Yin, Wei Li, Qin Li, Chong Qin, Zhi-Ping Zhang, Xiaowei Zhang, Xian-En Zhang*, Zongqiang Cui* (2018). Structural Characterization and Analysis of Human Immunodeficiency Virus Encapsulating Quantum Dots. Journal of Nanoscience and Nanotechnology, 18(7):4487-4494.

  16. Wenjing Zhang, Xian-En Zhang*, Feng Li* (2018).Virus-Based Nanoparticles of Simian Virus 40 in the Field of Nanobiotechnology. Biotechnology Journal, 13(6).

  17. Dong Men, Juan Zhou, Wei Li, Cui-Hua Wei, Yuan-yuan Chen, Kun Zhou, Ying Zheng, Ke Xu, Zhi-Ping Zhang, Xian-En Zhang* (2018). Self-Assembly of Antigen Proteins into Nanowires Greatly Enhances the Binding Affinity for High-Efficiency Target Capture. ACS Appl Mater Interfaces, 10(48):41019-41025.

  18. Yingxin Ma, Mingxiu Wang, Wei Li, Zhiping Zhang, Xiaowei Zhang, Guoqiang Wu, Tianwei Tan, Zongqiang Cui*, Xian-En Zhang* (2017). Live Visualization of HIV-1 Proviral DNA Using a Dual-Color-Labeled CRISPR System. Analytical Chemistry, 89(23): 12896-12901.

  19. Qin Li, Wei Li, Wen Yin, Jia Guo, Zhi-Ping Zhang, Dejun Zeng, Xiaowei Zhang, Yuntao Wu, Xian-En Zhang*, and Zongqiang Cui* (2017). Single-Particle Tracking of Human Immunodeficiency Virus Type 1 Productive Entry into Human Primary Macrophages. Acs Nano, 11(4):3890-3903.

  20. Yingxin Ma, Mingxiu Wang, Wei Li, Zhiping Zhang, Xiaowei Zhang, Tianwei Tan, Xian-En Zhang* & Zongqiang Cui* (2017). Live cell imaging of single genomic loci with quantum dot-labeled TALEs. Nature Communications, 8:15318.

  21. Kun Li, Ting Li, Shan-shan Yang, Xu-De Wang, Lei-xin Gao, Rui-Qi Wang, JingGu, Xian-En Zhang*, Jiao-Yu Deng* (2017). Deletion of nudB causes increased susceptibility to antifolates in Escherichia coli and Salmonella enterica. Antimicrobial Agents & Chemotherapy, 61(5):02378-16.

  22. Hua Li, Sha Wei, Yuan Fang, Min Li, Xia Li, Zhe Li, Jibin Zhang, Guofeng Zhu, Chuanyou Li, Lijun Bi, Guimin Zhang, Dianbing Wang,* and Xian-En Zhang* (2017).Quantitative proteomic analysis of host responses triggered by Mycobacterium tuberculosis infection in human macrophage cells. ABBS, 49 (9):835-844.

  23. Zhitao Zhang, Dianbing Wang, Chuanyou Li, Jiaoyu Deng, Jibin Zhang, Lijun Bi and Xian-En Zhang* (2017). Microbial sensor for drug susceptibility testing of Mycobacterium tuberculosis. J Appl. Microbiol, 124(1): 286-293.

  24. Jing Gu, Yuanyuan Chen, Hongsen Guo, Manluan Sun, Mingkun Yang, Xude Wang, Xian-En Zhang*, and Jiaoyu Deng* (2017). Lysine acetylation regulates the activity of Escherichia coil pyridoxine 5'-phosphate oxidase. ABBS, 49(2):186-192.

  25. 张先恩* (2017). 科技强国植根于深厚的基础研究.中国科学院院刊, 32(5):496-503.

  26. 张先恩* (2017). 生物传感发展50年及展望. 中国科学院院刊, 32(12):1271-1280.

  27. 王殿冰, 崔宗强, 张先恩* (2017). 分子生物传感器与细胞内分子影像. 中国科学院院刊, 32(12):1303-1312.

  28. 张先恩*, 刘云, 周程, 方在庆, 向桂林 (2017). 基础研究内涵及投入统计的国际比较. 中国软科学, (5):131-138.

  29. Xianxun Sun, Wei Li, Xiaowei Zhang, Mi Qi, Zhiping Zhang, Xian-En Zhang*, Zongqiang Cui* (2016). In Vivo Targeting and Imaging of Atherosclerosis Using Multifunctional Virus-Like Particles of Simian Virus 40. Nano Letters, 16:6164-6171.

  30. Yingxin Ma, Zhike He, Tianwei Tan, Wei Li, Zhiping Zhang, Shuang Song, Xiaowei Zhang, Qinxue Hu, Peng Zhou, Yuntao Wu, Xian-En Zhang* and Zongqiang Cui* (2016). Real-Time Imaging of Single HIV-1 Disassembly with Multicolor Viral Particles. ACS Nano, 10: 6273-6282

  31. Minghai Chen, Sanying Liu, Wei Li, Zhiping Zhang, Xiaowei Zhang, Xian-En Zhang*, Zongqiang Cui* (2016). Three-Fragment Fluorescence Complementation Coupled with Photoactivated Localization Microscopy for Nanoscale Imaging of Ternary Complexes. ACS Nano, 10: 8482-8490.

  32. Dong Men*, Juan Zhou, Wei Li, Yan Leng, Xinwen Chen, Sheng-ce Tao, Xian-En Zhang* (2016). Fluorescent Protein Nanowire-Mediated Protein Microarrays for Multiplexed and Highly Sensitive Pathogen Detection. ACS Applied Matrrials & Interfaces, 8:17472-17477.

  33. Xian-En Zhang*, Zongqiang Cui, Dianbing Wang (2016). Sensing of biomolecular interactions using fluorescence complementing systems in living cells. Biosensors & Bioelectronics, 76:243-250.

  34. Yingxin Ma, Zongqiang Cui, Zhike He, Wei Li, Zhi-Ping, Zhang, Xiaowei Zhang, Xian-En Zhang*, Tianwei Tan* (2016). Delaying Photobleaching of a Light-Switch Complex for Real-Time Imaging of Single Viral Particle Uncoating. Analytical Chemistry, 88:10675-10679.

  35. Ting Wang, Zhenhua Zheng, Xian-En Zhang*, Hanzhong Wang* (2016). Quantum dot-fluorescence in situ hybridisation for Ectromelia virus detection based on biotin-streptavidin interactions. Talanta, (2016)179–184.

  36. ZhaoYang Li, ZhiPing Zhang, LiJun Bi, ZongQiang Cui, JiaoYu Deng, DianBing Wang*, Xian-En Zhang* (2016). Mutagenesis of mNeptune Red-Shifts Emission Spectrum to 681-685 nm. PLOS ONE, 11(4):e0148749.

  37. D. Men, T. T. Zhang, L. W. Hou, J. Zhou, Z. P. Zhang, Y. Y. Shi, J. L. Zhang, Z. Q. Cui, J. Y. Deng, D. B. Wang and X. E. Zhang* (2015). Self-Assembly of Ferritin Nanoparticles into an Enzyme Nanocomposite with Tunable Size for Ultrasensitive Immunoassay. ACS Nano, 9(11): 10852-10860.

  38. X. L. Zhang, D. F. Li, J. Fleming, L. W. Wang, Y. Zhou, D. C. Wang, X. E. Zhang* and L. J. Bi* (2015). Core component EccB1 of the Mycobacterium tuberculosis type VII secretion system is a periplasmic ATPase. FASEB J, 29(12): 4804-4814.

  39. X. Y. Wang, D. B. Wang*, Z. P. Zhang, L. J. Bi, J. B. Zhang, W. Ding and X. E. Zhang* (2015). A S-Layer Protein of Bacillus anthracis as a Building Block for Functional Protein Arrays by In Vitro Self-Assembly. Small, 11(43): 5826-5832

  40. D. B. Wang, B. Tian, Z. P. Zhang, X. Y. Wang, J. Fleming, L. J. Bi, R. F. Yang and X. E. Zhang* (2015). Detection of Bacillus anthracis spores by super-paramagnetic lateral-flow immunoassays based on "Road Closure". Biosensors & Bioelectronics, 67: 608-614.

  41. M. Chen, W. Li, Z. Zhang, S. Liu, X. Zhang, X. E. Zhang* and Z. Cui* (2015). Novel near-infrared BiFC systems from a bacterial phytochrome for imaging protein interactions and drug evaluation under physiological conditions. Biomaterials, 48: 97-107.

  42. Q. Dong, J. Wang, H. Yang, C. Wei, J. Yu, Y. Zhang, Y. Huang, X. E. Zhang* and H. Wei* (2015). Construction of a chimeric lysin Ply187N-V12C with extended lytic activity against staphylococci and streptococci. Microb Biotechnol, 8(2): 210-220.

  43. S. Fan, D. F. Li, D. C. Wang, J. Fleming, H. Zhang, Y. Zhou, L. Zhou, J. Zhou, T. Chen, G. Chen, X. E. Zhang* and L. Bi* (2015). Structure and function of Mycobacterium smegmatis 7-keto-8-aminopelargonic acid (KAPA) synthase. Int J Biochem Cell Biol, 58, 71-80.

  44. S. H. Fan, Y. Zhou, H. T. Zhang, J. Fleming, Z. N. Yu, X. E. Zhang* and L. J. Bi* (2015). MutM Interaction Partners Detected in Mycobacterium smegmatis by Tandem Affinity Purification. Progress in Biochemistry and Biophysics, 42(10): 935-946.

  二、非通讯作者论文(近三年发表)

  1. Yingxin Ma, Guobin Mao, Weiren Huang, Guoqiang Wu, Wen Yin, Xinghu Ji, Zishi Deng, Zhiming Cai, Xian-En Zhang, Zhike He, Zongqiang Cui* (2019). Quantum Dot Nanobeacons for Single RNA Labeling and Imaging. Journal of the American Chemical Society, 141(34):13454-13458.

  2. Chong Qin, Wei Li, Qin Li, Wen Yin, Xiaowei Zhang, Zhi-Ping Zhang, Xian-En Zhang, Zongqiang Cui* (2019). Real-time dissection of dynamic uncoating of individual influenza viruses. PNAS, 116(7):2577-2582.

  3. Qin Li, Wen Yin, Wei Li, Zhi-Ping Zhang, Xiaowei Zhang, Xian-En Zhang, Zongqiang Cui* (2018). Encapsulating Quantum Dots within HIV-1 Virions through Site-Specific Decoration of the Matrix Protein Enables Single Virus Tracking in Live Primary Macrophages. Nano Letters, 18(12):7457-7468.

  4. Juan Zhou, Jinxiang Duan, Xian-En Zhang, Qiang Wang*, Dong Men* (2018). A chiral responsive carbon dots-gold nanoparticle complex mediated by hydrogen peroxide independent of surface modification with chiral ligands. Nanoscale, 10(39):18606-18612.

  5. Minghai Chen, Wei Li, Zhi-Ping Zhang, Jingdi Pan, Yuhan Sun, Xiaowei Zhang, Xian-En Zhang, Zongqiang Cui* (2018). Three-Fragment Fluorescence Complementation for Imaging of Ternary Complexes under Physiological Conditions. Analytical Chemistry, 90(22):13299-13305.

  6. Mi Qi, Xian-En Zhang, Xianxun Sun, Xiaowei Zhang, Yanfeng Yao, Siling Liu, Ze Chen, Wei Li, Zhi-Ping Zhang, Jianjun Chen*, Zongqiang Cui* (2018). Intranasal Nanovaccine Confers Homo- and Hetero-Subtypic Influenza Protection. Small, 14(13).

  7. Fanlin Wu, Yin Liu, Hai-Nan Zhang, He-Wei Jiang, Li Cheng, Shu-Juan Guo, Jiaoyu Deng, Li-jun Bi, Xian-En Zhang, Hua-Fang Gao*, Sheng-Ce Tao* (2018).Global Profiling of PknGInteractions Using a Human Proteome Microarray Reveals Novel Connections with CypA. Proteomics, 18(23).

  8. He-Wei Jiang, Daniel M. Czajkowsky, Tao Wang, Xu-De Wang, Jia-Bin Wang, Hai-Nan Zhang, Cheng-Xi Liu, Fanlin Wu, Xiang He, Zhaowei Xu, Hong Chen, Shujuan Guo, Yang Li, Li-Jun Bi, Jiaoyu Deng, Jin Xie, Jian-Feng Pei, Xian-En Zhang, Sheng-Ce Tao* (2018). Identification of Serine 119 as an Effective Inhibitor Binding Site of M. tuberculosis Ubiquitin-like Protein Ligase PafA Using Purified Proteins and M. smegmatis. Ebiomedicine, 30:225-236. 

  9. Kun Li, Xu-De Wang, Shanshan Yang, Jing Gu, Jiaoyu Deng*, Xian-En Zhang (2017). Anti-folates potentiate bactericidal effects of other antimicrobial agents. Journal of Antibiotics, 70(3):285-291.

  10. Hainan Zhang, Zhaowei Xu, Hewei Jiang, Fanlin Wu, Xiang He, Yin Liu, Shujuan Guo, Yang Li, Li-Jun Bi, Jiaoyu Deng, Xian-En Zhang, Sheng-Ce Tao* (2017). Cyclic di-GMP regulates Mycobacterium tuberculosis resistance to ethionamide. Scientific Reports, 7.

  11. DShanshan Yang, Yang-Bo Hu, Xu-De Wang, Yun-Rong Gao, Kun Li, Xian-En Zhang, Shiyun Chen, Tian-Yu Zhang, Ling Gu*, Jiaoyu Deng* (2017). Deletion of sigB Causes Increased Sensitivity to para-Aminosalicylic Acid and Sulfamethoxazole in Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy, 61(10).

  12. Thaer Kadhim Fauuadh, Fuying Ma, Chong Qin, Xiaowei Zhang, Wei Li, Xian-En Zhang, Zhiping Cui, Zongqiang Cui* (2017). Simultaneous detection of multiple viruses in their co-infected cells using multicolour imaging with self-assembled quantum dot probes. Microchimica Acta, 184(8):2815-2824.

  13. Yun-Rong Gao, Defeng Li, Joy Flemming, Yafeng Zhou, Ying Liu, Jiaoyu Deng, Lin Zhou, Jie Zhou, Guofeng Zhu, Xian-En Zhang, Dacheng Wang*, Li-Jun Bi* (2017). Structural analysis of the regulatory mechanism of MarR protein Rv2887 in M-tuberculosis. Scientific Reports, 7.

  14. Xiang He, Hewei Jiang, Hong Chen, Hainan Zhang, Yin Liu, Zhaowei Xu, Fanlin Wu, Shujuan Guo, Jingli Hou, Mingkun Yang, Wei Yan, Jiaoyu Deng, Lijun Bi, Xian-En Zhang, Shengce Tao* (2017). Systematic Identification of Mycobacterium tuberculosis Effectors Reveals that BfrB Suppresses Innate Immunity. Molecular & Cellular Proteomics, 16(12):2243-2253.

  15. Fanlin Wu, Yin Liu, Hewei Zhang, Yizhao Luan, Hainan Zhang, Xiang He, Zhaowei Xu, Jingli Hou, Liyun Ji, Zhi Xie, Daniel M. Czajkowsky, Wei Yan, Jiaoyu Deng, Lijun Bi, Xian-En Zhang, Shengce Tao* (2017). The Ser/Thr Protein Kinase Protein-Protein Interaction Map of M-tuberculosis. Molecular & Cellular Proteomics, 16(8):1491-1506. 

  

  资料来源:张先恩研究员,2019-11-29网页更新 

           
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